Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum Pox Potyvirus
Identifieur interne : 000221 ( France/Analysis ); précédent : 000220; suivant : 000222Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum Pox Potyvirus
Auteurs : Marthe Brison [France] ; Marie-Thérèse De Boucaud [France] ; André Pierronnet [France] ; Françoise Dosba [France]Source :
- Plant Science [ 0168-9452 ] ; 1997.
Abstract
An in vitro grown interspecific Prunus rootstock, Fereley-Jaspi (R), which had been infected by chip budding with the Plum Pox Potyvirus Marcus strain, was used as experimental material. After excision from Fereley plantlets which had been subcultured 7, 14, 30 days, shoot tips were sorted by size (from 0.3 to 2 mm). Control shoot tips were grown directly on meristem culture medium. Experimental shoot tips were cultured 24 h at 4°C on a preculture medium enriched with dimethylsulfoxide and proline, then treated with modified PVS2 cryoprotective solution and cooled at 1°C/min to −40°C before immersion in liquid nitrogen. When plantlets, from both, control and cryopreserved shoot tips were 4 month old, they were tested by the immunocapture Polymerase Chain Reaction method (IC-PCR) for Plum Pox virus detection. An average of 50 and 20%, respectively for frozen and control shoot tips were virus free. The impact of cryopreservation on producing virus-free plantlets was also studied in relation to shoot tip size and to the duration of subculture of the plantlets before excision of the shoot tips. Both of these parameters influenced the percentage of virus-free plantlets obtained from control shoot tips, but not that obtained from cryopreserved ones.
Url:
DOI: 10.1016/S0168-9452(97)04581-0
Affiliations:
- France
- Aquitaine, Languedoc-Roussillon, Nouvelle-Aquitaine, Occitanie (région administrative)
- Montpellier, Talence, Villenave d'Ornon
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000853
- to stream Istex, to step Curation: 000853
- to stream Istex, to step Checkpoint: 000C89
- to stream Main, to step Merge: 003559
- to stream Main, to step Curation: 003312
- to stream Main, to step Exploration: 003312
- to stream France, to step Extraction: 000221
Links to Exploration step
ISTEX:8D6554AFDC07277C4FD6DC4A33EE6D70D04061D2Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum Pox Potyvirus</title>
<author><name sortKey="Brison, Marthe" sort="Brison, Marthe" uniqKey="Brison M" first="Marthe" last="Brison">Marthe Brison</name>
</author>
<author><name sortKey="De Boucaud, Marie Therese" sort="De Boucaud, Marie Therese" uniqKey="De Boucaud M" first="Marie-Thérèse" last="De Boucaud">Marie-Thérèse De Boucaud</name>
</author>
<author><name sortKey="Pierronnet, Andre" sort="Pierronnet, Andre" uniqKey="Pierronnet A" first="André" last="Pierronnet">André Pierronnet</name>
</author>
<author><name sortKey="Dosba, Francoise" sort="Dosba, Francoise" uniqKey="Dosba F" first="Françoise" last="Dosba">Françoise Dosba</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:8D6554AFDC07277C4FD6DC4A33EE6D70D04061D2</idno>
<date when="1997" year="1997">1997</date>
<idno type="doi">10.1016/S0168-9452(97)04581-0</idno>
<idno type="url">https://api.istex.fr/document/8D6554AFDC07277C4FD6DC4A33EE6D70D04061D2/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000853</idno>
<idno type="wicri:Area/Istex/Curation">000853</idno>
<idno type="wicri:Area/Istex/Checkpoint">000C89</idno>
<idno type="wicri:doubleKey">0168-9452:1997:Brison M:effect:of:cryopreservation</idno>
<idno type="wicri:Area/Main/Merge">003559</idno>
<idno type="wicri:Area/Main/Curation">003312</idno>
<idno type="wicri:Area/Main/Exploration">003312</idno>
<idno type="wicri:Area/France/Extraction">000221</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum Pox Potyvirus</title>
<author><name sortKey="Brison, Marthe" sort="Brison, Marthe" uniqKey="Brison M" first="Marthe" last="Brison">Marthe Brison</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Physiologie Cellulaire Végétale, Université Bordeaux 1, Avenue des Facultés, 33405 Talence</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Talence</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="De Boucaud, Marie Therese" sort="De Boucaud, Marie Therese" uniqKey="De Boucaud M" first="Marie-Thérèse" last="De Boucaud">Marie-Thérèse De Boucaud</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Physiologie Cellulaire Végétale, Université Bordeaux 1, Avenue des Facultés, 33405 Talence</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Talence</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pierronnet, Andre" sort="Pierronnet, Andre" uniqKey="Pierronnet A" first="André" last="Pierronnet">André Pierronnet</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>INRA, Unité de Recherche sur les Espèces Fruitières et la Vigne, BP 81, 33883 Villenave d'Ornon cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Villenave d'Ornon</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dosba, Francoise" sort="Dosba, Francoise" uniqKey="Dosba F" first="Françoise" last="Dosba">Françoise Dosba</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>ENSAM, Unité de Formation et de Recherche d'Arboriculture Fruitière, 2 place Viala, 34060 Montpellier cedex 1</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Plant Science</title>
<title level="j" type="abbrev">PSL</title>
<idno type="ISSN">0168-9452</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1997">1997</date>
<biblScope unit="volume">123</biblScope>
<biblScope unit="issue">1–2</biblScope>
<biblScope unit="page" from="189">189</biblScope>
<biblScope unit="page" to="196">196</biblScope>
</imprint>
<idno type="ISSN">0168-9452</idno>
</series>
<idno type="istex">8D6554AFDC07277C4FD6DC4A33EE6D70D04061D2</idno>
<idno type="DOI">10.1016/S0168-9452(97)04581-0</idno>
<idno type="PII">S0168-9452(97)04581-0</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0168-9452</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">An in vitro grown interspecific Prunus rootstock, Fereley-Jaspi (R), which had been infected by chip budding with the Plum Pox Potyvirus Marcus strain, was used as experimental material. After excision from Fereley plantlets which had been subcultured 7, 14, 30 days, shoot tips were sorted by size (from 0.3 to 2 mm). Control shoot tips were grown directly on meristem culture medium. Experimental shoot tips were cultured 24 h at 4°C on a preculture medium enriched with dimethylsulfoxide and proline, then treated with modified PVS2 cryoprotective solution and cooled at 1°C/min to −40°C before immersion in liquid nitrogen. When plantlets, from both, control and cryopreserved shoot tips were 4 month old, they were tested by the immunocapture Polymerase Chain Reaction method (IC-PCR) for Plum Pox virus detection. An average of 50 and 20%, respectively for frozen and control shoot tips were virus free. The impact of cryopreservation on producing virus-free plantlets was also studied in relation to shoot tip size and to the duration of subculture of the plantlets before excision of the shoot tips. Both of these parameters influenced the percentage of virus-free plantlets obtained from control shoot tips, but not that obtained from cryopreserved ones.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Aquitaine</li>
<li>Languedoc-Roussillon</li>
<li>Nouvelle-Aquitaine</li>
<li>Occitanie (région administrative)</li>
</region>
<settlement><li>Montpellier</li>
<li>Talence</li>
<li>Villenave d'Ornon</li>
</settlement>
</list>
<tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Brison, Marthe" sort="Brison, Marthe" uniqKey="Brison M" first="Marthe" last="Brison">Marthe Brison</name>
</region>
<name sortKey="De Boucaud, Marie Therese" sort="De Boucaud, Marie Therese" uniqKey="De Boucaud M" first="Marie-Thérèse" last="De Boucaud">Marie-Thérèse De Boucaud</name>
<name sortKey="Dosba, Francoise" sort="Dosba, Francoise" uniqKey="Dosba F" first="Françoise" last="Dosba">Françoise Dosba</name>
<name sortKey="Pierronnet, Andre" sort="Pierronnet, Andre" uniqKey="Pierronnet A" first="André" last="Pierronnet">André Pierronnet</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000221 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000221 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= France |étape= Analysis |type= RBID |clé= ISTEX:8D6554AFDC07277C4FD6DC4A33EE6D70D04061D2 |texte= Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum Pox Potyvirus }}
This area was generated with Dilib version V0.6.25. |